Design of primers and probes using primer express software. Primer express software download thermo fisher scientific uk. One way, of course, is to do all the bioinformatics work yourself on the context sequence, then use a program such as primer express software to. Ive had great success for rtqpcr primer design with both oligoarchitect. This section describes the guidelines for designing taqman mgb probes using primer. Primer express software allows you to design your own primers and probes using taqman and sybr green i dye chemistries for gene quantitation and allelic. Ongoing evolution of viral pathogens is a significant issue in diagnostic virology employing taqman qpcrrtqpcr. Primer and probe design using the primer express software. Beacon designer free edition is a free online tool for analyzing sybr green primers and taqman probes.
Applied biosystems, primer express, abi prism are registered trademarks. Hints for realtime pcr qpcr assay design ask taqman. Primer express software download thermo fisher scientific us. Selection of the appropriate reagent configuration taqman universal pcr. Applied biosystems realtime pcr rapid assay development. Allows users to design targetspecific primers for realtime pcr qpcr experiments. I am working on primer and probe design for realtime pcr assay. Designing taqman mgb probe and primer sets for gene. Sequence detection systems quantitative assay design and. I am looking for the best way of designing probes for. The software allows for parameter modification in its automatic design feature, but is lacking any sort of parameter to lock a. Specific concerns are related to false negativity due to probe binding failure.